Lysolecithin Metabolism by Human Platelets

Lysolecithin metabolism by human platelets.

Human platelets incubated with 32p lysolecithin convert this compound to platelet lecithin and the water-soluble product glycerylphosphorylcholine. Lecithin synthesis from lysolecithin by intact platelets has a pH optimum of 9, whereas platelet homogenates show a pH optimum of 7.5 for the same reaction. Conversion of lysolecithin to platelet lecithin takes place by direct acylation of lysolecit...

Metabolism of Exogenous Diacylglycerols by Human Platelets*

The metabolism of exogenous [3H]diacylglycerols by intact human platelets was studied in order to examine: 1) the metabolic fate of these second messengers in an intact cell, 2) the effect of diacylglycerol kinase and diacylglycerol lipase inhibitors on this metabolism, 3) the effect of agonist stimulation on metabolism, and 4) the dependence of metabolism on diacylglycerol chain length. When 2...

Lipid metabolism in human platelets.

Induction of apoptosis in human tumor cell lines by platelets

Introduction: It has been reported that platelets can eradicate tumor cells in vitro, although the mechanism of this effect has not been determined. The effect of platelets on the induction of apoptosis in tumor cells is largely unknown. Materials and methods: To investigate this effect, two human hematologic cell lines, K562 and Daudi, were independently faced with unstimulated and thromb...

Metabolism of dehydroepiandrosterone sulfate and estrone-sulfate by human platelets.

The aim of the present research was to study the uptake of DHEAS, and to establish the intracrine capacity of human platelets to produce sex steroid hormones. The DHEAS transport was evaluated through the uptake of [(3)H]-DHEAS in the presence or absence of different substrates through the organic anion transporting polypeptide (OATP) family. The activity of sulfatase enzyme was evaluated, and ...